Molecular characterisation and disease severity of leptospirosis in Sri Lanka

Leptospirosis is a re-emerging zoonotic disease all over the world, important in tropical and subtropical areas. A majority of leptospirosis infected patients present as subclinical or mild disease while 5-10% may develop severe infection requiring hospitalisation and critical care. It is possible that several factors, such as the infecting serovar, level of leptospiraemia, host genetic factors and host immune response, may be important in predisposition towards severe disease. Different Leptospira strains circulate in different geographical regions contributing to variable disease severity. Therefore, it is important to investigate the circulating strains at geographical locations during each outbreak for epidemiological studies and to support the clinical management of the patients. In this study immunochromatography, microscopic agglutination test and polymerase chain reaction were used to diagnose leptospirosis. Further restriction fragment length polymorphism and DNA sequencing methods were used to identify the circulating strains in two selected geographical regions of Sri Lanka. Leptospira interrogans, Leptospira borgpetersenii and Leptospira kirschneri strains were identified to be circulating in western and southern provinces. L. interrogans was the predominant species circulating in western and southern provinces in 2013 and its presence was mainly associated with renal failure.

Spinal tuberculosis; A study of the disease pattern, diagnosis and outcome of medical management in Sri Lanka.

Background: Sri Lanka has an intermediate burden of tuberculous disease. Most patients with spinal tuberculosis (STB) are managed with medical treatment alone as advanced surgical facilities are not freely available. Objective: To describe the clinico-demographic and imaging pattern of STB and to assess the outcome of medical treatment in the local setting. Design: Descriptive case series. Methods: All patients diagnosed with definite or probable STB, had their symptomatology and investigations recorded. They were followed up with anti-TB treatment (ATT) according to standard guidelines. An initial six-week tapering course of steroid was given when there was an evidence of neural involvement. Results: Of 32 patients with STB, backache was the commonest presenting feature (92%). Nine had lower limb neurological deficits. Uni-focal upper lumbar involvement was the commonest disease pattern noted in the series. High ESR (84%) and Mantoux positivity (53%) were frequent. 72% had end-plate changes on imaging. 53% had paraspinal soft tissue components. The triad of backache, high ESR and end-plate and/or paraspinal disease on CT/MRI showed a diagnostic sensitivity of 81.2%. Response to ATT was satisfactory in 87%. Poor neurological response was seen among some with large paraspinal collections or extensive vertebral damage at diagnosis. Conclusion: This study showed that backache over one month, high ESR and specific CT/MRI features helped diagnosis of STB, in the absence of definitive evidence. Medical management alone, comprising a prolonged course of ATT with an initial steroid cover when indicated, appeared to be safe and effective in the local setting for uncomplicated STB.

An assessment of the effectiveness of tobacco control measures on behavior changes related to tobacco use among adolescents and young adults in a district in Sri Lanka.

Introduction: Sri Lanka became a signatory to the WHO Frame Work Convention on Tobacco Control in September 2003, and this was ratified in November 2003. With a view to reduce the use of tobacco in Sri Lanka, the National Authority on Tobacco and Alcohol Act (NATA) No. 27 of 2006 was implemented. Aim: To assess the behavior changes related to tobacco use among adolescents and young adults following exposure to tobacco control measures were implemented by NATA. Materials and Methods: A case-control study was conducted on 42 adolescent (aged 13-19 years) and 156 young adult (aged 20-39 years) men living in Anuradhapura Divisional Secretary area in Sri Lanka. Cases (current quitters) and controls (current smokers) were compared to ascertain the outcome following the exposure to tobacco control measures. A self-administered questionnaire and focus group discussions were used to ascertain the exposure status in cases and controls. Confounding was controlled by stratification and randomization. Univariate analysis was performed by Backward Stepwise (Likelihood Ratio) method. Results: Among 198 respondents, 66 (27.3% adolescents and 72.7% young adults) were quitters, while 132 smokers (18.2% adolescents and 81.8% young adults) were current smokers. Exposure to the anti-smoking media messages revealed that TV was the strongest media that motivated smokers to quit smoking. Majority (66%) of cases and control were not exposed to tobacco promotion advertisements, while 47% of the cases and 50% of the control had never seen tobacco advertisements during community events. All cases (66) as well as 89% (118) of the control had not noticed competitions or prizes sponsored by tobacco industry during last year ( P = 0.13). Conclusion: Tobacco control measures implemented by NATA had a favorable influence on behavior change related to smoking among quitters and current smokers.

Smokeless tobacco use in Sri Lanka.

To comprehensively review the issues of smokeless tobacco use in Sri Lanka . This review paper is based on a variety of sources including Medline, WHO documents, Ministry of Health and Nutrition, Colombo and from other sources. Results: The prevalence of smokeless tobacco (SLT) use in Sri Lanka has been reported high, especially among rural and disadvantaged groups. Different smokeless tobacco products were not only widely available but also very affordable. An increasing popularity of SLT use among the youth and adolescents is a cause for concern in Sri Lanka. There were evidences of diverse benign, premalignant, and malignant oral diseases due to smokeless tobacco use in the country. The level of awareness about health risks related to the consumption of smokeless tobacco products was low, particularly among the people with low socio-economic status. In Sri Lanka various forms of smokeless tobacco products, some of them imported, are used. At the national level, 15.8% used smokeless tobacco products and its use is three-fold higher among men compared to women. Betel quid is by far the traditional form in which tobacco is a general component. Other manufactured tobacco products include pan parag/pan masala, Mawa, Red tooth powder, Khaini, tobacco powder, and Zarda. Some 8.6% of the youth are current users of smokeless tobacco. There are studies demonstrating the harmful effects of smokeless tobacco use, especially on the oral mucosa, however, the level of awareness of this aspect is low. The highest mean expenditure on betel quid alone in rural areas for those earning Rs. 5,000/month was Rs. 952. The core issue is the easy availability of these products. To combat the smokeless tobacco problem, public health programs need to be intensified and targeted to vulnerable younger age groups. Another vital approach should be to levy higher taxation.

On the variations of the muscle flexor digitorum brevis: anatomical insight / Variaciones del músculo flexor corto de los dedos: experiencias de anatomía

Flexor digitorum brevis, an intrinsic muscle of the sole of the foot, is stated as one of the muscles that shows frequent anatomical variations. Its most commonly reported anomaly is the absence of tendon to the little toe. From a clinical standpoint of view, flexor digitorum brevis musculocutanous flap is used in the reconstruction of the heel pad and flexor digitorum brevis tendon transfer is employed in the correction of flexible claw and hammer toe deformities. Despite its significance little is known regarding the variations of this muscle in Sri Lankans. Hence, the goal of this study was to elucidate the incidence and morphological features of this muscle in an adult Sri Lankan population. A total of one hundred and thirty five apparently healthy human feet were dissected and studied for the morphological features of the flexor digitorum brevis muscle. The incidence of the absent fourth tendon of the flexor digitorum brevis in the present study was found to be 71.85 percent. In all cases, when absent, it was missing bilaterally. The results of the present study further highlight the anatomical variations of the flexor digitorum brevis muscle. A sound knowledge about the anatomical variations of flexor digitorum brevis will facilitate the outcome of surgical as well as diagnostic imaging techniques of the foot. Furthermore, understanding muscle architecture of the foot may assist in the design of prosthesis and analysis of foot function.

El músculo flexor corto de los dedos, es un músculo intrínseco de la planta del pie que frecuentemente presenta variaciones anatómicas. La variación más frecuente es la ausencia de tendón destinado al dedo mínimo. Desde el punto de vista clínico se utilizan colgajos musculocutáneos del músculo flexor corto de los dedos para la reconstrucción de la región subcalcánea. Además, el tendón del músculo es usado para corregir deformidades de garra y martillo de los dedos del pie. A pesar de su importancia, es escasa la información acerca de este músculo en la población adulta de Sri Lanka.Fueron disecados 135 pies humanos aparentemente sanos analizándose sus características morfológicas. Se determinó que un 71,85 por ciento el tendón para el quinto dedo del músculo flexor corto de los dedos estaba ausente. En todos los casos, ésta se presentaba bilateralmente. El estudio señala la importancia de tener un cabal conocimiento acerca de las variaciones anatómicas del músculo flexor corto de los dedos, para facilitar tanto las técnicas quirúrgicas, como el diagnostico a través de imagenlogía del pie. Además, un buen conocimiento de la arquitectura muscular del pie puede ayudar en el diseño de prótesis y en el análisis de la función del pie.

A Sri Lankan child with 49,XXXXY syndrome.

Pentasomy 49,XXXXY is a rare sex chromosome disorder usually presenting with ambigous genitalia, facial dysmorphism, mental retardation and a combination of cardiac, skeletal and other malformations. The incidence of the condition is estimated to be 1 in 85,000 male births. Previously, this condition was identified as a Klinefelter variant. The condition is suspected in a patient, by a combination of characteristic clinical findings, and the diagnosis is confirmed by chromosome culture and karyotyping. In the case we report here, the main presentation of ambiguous genitalia led to a suspicion of a sex chromosome aneuploidy which was subsequently confirmed by chromosomal analysis.

Prevalence and predictors of default with tuberculosis treatment in Sri Lanka.

The objectives of this study were to determine the default rate and predictors for default in patients undergoing antituberculosis treatment. All consenting patients with a confirmed diagnosis of tuberculosis admitted to a unit of the Chest Hospital, Welisara, Sri Lanka from April 2001 to April 2002 were recruited into the study. Personal and follow-up data were recorded on a pre-tested questionnaire and data sheet, respectively. A defaulter was defined as a patient who interrupted treatment for more than two consecutive months before the end of the course of treatment. Of the 892 patients recruited, 770 were new cases and 122 were relapses. The default rates were 10.3% (95% CI: 8.3-12.6) and 30.3% (95% CI: 22.7-38.1) among new cases and retreatment cases, respectively, during the intensive phase of treatment and 10.9% (95% CI:8.7-13.3) and 16.5% (95% CI:9.7-25.5), respectively, during the continuation phase. Ninety percent of new cases and 94% of retreatment cases were sputum positive for acid-fast bacilli at diagnosis. Two hundred five patients (22.9%) defaulted on treatment (95% CI: 20.3-25.8). Using logistic regression analysis, regular smokers (OR = 1.9), smear positive patients who were previous defaulters (OR = 2.4) and patients having involvement of less than 3 zones of the lung on chest x-ray (OR = 0.5) were more likely to default compared to patients who did not smoke regularly, smear positive patients who had relapsed after taking the full course of treatment and patients with less lung involvement. Skilled and unskilled laborers were the most likely occupation to default (OR = 2.03) followed by sales personnel (OR = 2.00), compared to the unemployed or home-bound. A high default rate of 23% was observed among the study participants. Smoking status, occupation, history of treatment compliance of the patient, and extent of lung involvement were predictors for defaulting.

Assessment of body composition in Sri Lankan children: validation of a skin fold thickness equation.

BACKGROUND: Morbidity associated with obesity is related to the fat mass (FM) of the body. The direct estimation of FM is difficult. Skin fold thickness (SFT) is a simple and cheap alternative for accurate assessment of FM, and population specific equations are necessary for accurate assessment of FM using SFT. OBJECTIVE: To develop a SFT prediction equation to estimate FM of Sri Lankan children. DESIGN, SETTING AND METHOD: Across-sectional descriptive study was done at the University Paediatric Unit of Lady Ridgeway Hospital, Colombo. Data were collected from 5 to 15 year old healthy children. Triceps, biceps, supra-iliac and subscapular SFT were measured using Harpendens skin fold caliper. Total body water was assessed using an isotope dilution method (D2O), and fat free mass calculated. FM was assessed based on 2 compartment body composition model. Multiple regression analysis was used to develop prediction equation and validated using PRESS (prediction of sum of squares) statistical technique. Independent variables were age, triceps SFT, subscapular SFT and sex. RESULTS: Prediction equation for FM [(0.68 x age) + (0.246 x triceps SFT) + (0.383 x subscapular SFT) - (1.61 x sex code) -3.45] was able to predict 76.4% of variance with a root mean squared error (RMSE) of 3.4 kg. PRESS statistics was 3.4 kg with press residuals of 1.56 kg. Bland-Altman technique showed that the majority of the residuals were within mean bias +/-1.96 SD. CONCLUSION: Results of this study provide an SFT equation for the prediction of FM in Sri Lankan children.

A case of imported malaria: the first report of a Plasmodium malariae infection in Sri Lanka after 37 years.

We report a case of Plasmodium falciparum and P. malariae mixed infection in a patient who had been living in Malawi. This is the first case of P. malariae reported in Sri Lanka in 4 decades. The presence of both parasites was confirmed by microscopy and polymerase chain reaction (PCR). The history strongly indicated that the infection had been acquired from Malawi. The patient had liver dysfunction and a transient glomerulonephritis, both of which subsided with antimalarial treatment.

Prevalence of colorectal cancer and survival in patients from the Gampaha District, North Colombo region.

INTRODUCTION: The prevalence and survival of colorectal cancer in Sri Lankans has not been previously reported. We did a retrospective and a prospective survey, in the region of North Colombo, Sri Lanka between 1992 and 2004. The aim was to study cancer burden, sites of colorectal cancer and survival after surgery. PATIENTS AND METHODS: The records of 175 patients with colorectal cancer between 1992 and 1997 in the selected region of were analysed retrospectively. A prospective study was performed in 220 new patients with colorectal cancer between 1996 and 2004. Data evaluated were demographics, tumour stage and survival. RESULTS: Between 1992 and 1997 the crude annual incidence of colorectal cancer was 1.9 per 100,000, which increased over the years. The current national crude annual incidence is 3.2 per 100,000 in women and 4.9 in men. Median age at presentation was 60 years with similar prevalence of cancer in men and women. In the entire group, 28% of cancers were in those less than 50 years old. Survival at 2 and 5 years was 69% and 52%. The majority of cancer related deaths were within the first 2 years after surgery. CONCLUSION: The burden of colorectal cancer in Sri Lanka is on the rise. Up to a third of cancers occur in those under 50 years, and the majority of cancers are in the rectum or rectosigmoid region. Flexible sigmoidoscopy offers a useful screening tool.

Effect of repeated mass chemotherapy for filariasis control on soil-transmitted helminth infections in Sri Lanka.

BACKGROUND: In July 2006 Sri Lanka completed 5 rounds of annual mass drug administration (MDA) with diethylcarbamazine citrate (DEC) and albendazole as part of its national programme for elimination of lymphatic filariasis (LF). Albendazole is highly effective against soil-transmitted helminths (STH). This study was carried out to assess the effect of repeated annual MDA on STH infections in the Western Province of Sri Lanka, an area co-endemic for LF and STH. METHODS: Faecal samples were obtained (during August-September 2006), from grade 5 students in 17 schools in the Western Province that were included in a national survey of schoolchildren's health in 2003, and examined using the modified Kato-Katz technique. The prevalence and intensity of roundworm, whipworm and hookworm infections in 2003 and 2006 were compared. RRESULTS:Faecal samples from 255 children were examined in 2003; 448 were examined in 2006. Roundworm prevalence was marginally lower in 2006 (4.0%) than in 2003 (4.7%), as was hookworm (0.2% vs 0.4%), whereas whipworm prevalence was higher (13.8% vs 9.4%). These differences as well as that between the geometric mean egg counts were not statistically significant. Compliance with MDA in 2006, as reported by the schoolchildren examined, was only 59%. CCONCLUSIONS:Four annual rounds of MDA with DEC and albendazole had virtually no effect on STH infections in the study area.

Hormone receptor expression and Her/2neu amplification in breast carcinoma in a cohort of Sri Lankans.

OBJECTIVE: 1) To determine the profile of estrogen and, progesterone receptors (ER, PR) expression and Her/2neu amplification in carcinoma of breast of Sri Lankan women. 2) To determine their inter-relationships, and associations with age at diagnosis and histological grade. DESIGN: Retrospective analysis of data. SETTING: Department of Pathology, Faculty of Medicine, University of Peradeniya. MATERIALS: 124 samples of invasive ductal carcinoma of breast, stained for steroid receptors and Her/2neu amplification with immunohistochemistry. MEASUREMENTS: 1) Semiquantative scores of steroid receptors and Her/2neu amplification. 2) Correlations of ER, PR, Her/2neu amplification, age at diagnosis and histological grade. RESULTS: The prevalence of ER, PR and Her/2neu amplification were 53.2%, 50% and 14.6% respectively. The expression of ER and PR were significantly correlated (p < 0.001). and had a significant negative correlation with Her/2neu amplification (p0.003 each). Lower grade of the tumour was significantly related to the expression of ER (p0.000) and PR (p0.000) but not to Her/2neu amplification (p0.331). Age at diagnosis was significantly correlated to the expression of ER (p0.004), but not to PR (p0.365) or Her/2neu amplification (p0.456). CONCLUSION: Prevalence or ER, PR and Her/2neu amplification in carcinoma of breast among Sri Lankans is similar to that described internationally. The correlations of ER, PR, Her/2neu amplification, to each other, age at diagnosis and grade of tumour is as reported in other countries.

Epidemiological aspects and risk factors of toxocariasis in a pediatric population in Sri Lanka.

This cross-sectional study, carried out over a period of 11 months, investigated the relationship between Toxocara seropositivity, socio-demographic and environmental variables in a pediatric population. Risk factors for Toxocara infection were assessed by direct interview of parent or guardian using a structured pre-tested questionnaire. Eosinophilia and presence of helminth eggs or protozoan cysts in a fecal smear were recorded. Diagnosis of Toxocara seropositivity in children was based on IgG Toxocara Microwell Serum Elisa Kits. The ELISA test was regarded as positive if the optical density was 0.3 units or above. Unadjusted and adjusted odds ratios were calculated to determine risk factors for disease. The proportion of children who were positive for Toxocara antibodies in the study population was 20%. Children being exposed to a puppy of less than 3 months at home, visiting a playground frequently, living in a poorly constructed house and dogs having access to playgrounds were significant risk factors on univariate analysis. Of these four variables, only the first three variables (OR 19, OR 4 and OR 3, respectively) remained significant risk factors on the multivariate model. Presence of eosinophilia in seropositive children was significantly higher than the seronegative group (77% vs 40%; p < 0.001). This study indicates that dogs contribute significantly to children being seropositive for toxocariasis in Sri Lanka. Implementation of public health programs specifically focused on anti-parasitic treatment of dogs is recommended.

The social determinants of childhood mortality in Sri Lanka: timetrends & comparisons across South Asia.

BACKGROUND & OBJECTIVE: Sri Lanka has been able to achieve low childhood mortality levels at low cost. However, this achievement may have been at the expense of increasing mortality inequalities between socio-economic groups. This study addresses the question whether socio-economic mortality inequalities rise as overall mortality falls by describing socio-economic inequalities in under 5 mortality in Sri Lanka and comparing the magnitude of these inequalities over time and with other South Asian countries. Further, the role of female autonomy, fertility, malnutrition, and health care use in explaining the observed patterns in mortality inequality were also examined. METHODS: Time-trends in inequality in under 5 mortality by maternal education were described using data from the 1987, 1993 and 2000 Sri Lanka Demographic and Health Surveys (DHS). Using DHS data, the magnitude of these inequalities was compared across 50 low and middle income countries, and with three South Asian countries in particular. Socio-economic inequalities in determinants of under 5 mortality were estimated for Sri Lanka over time, and compared with such inequalities in the other South Asian countries. RESULTS: Absolute inequalities in under 5 mortality in Sri Lanka were very low internationally, while relative mortality inequalities were high. The decline in under 5 mortality between the 1987 and 2000 survey in Sri Lanka had been accompanied by rising relative mortality inequalities across educational groups. High and improving levels of health care use and declining levels of malnutrition in Sri Lanka ran parallel with high and increasing relative inequalities in undercoverage of health care and malnutrition. INTERPRETATION & CONCLUSION: Despite the low overall under 5 mortality levels and absolute mortality inequalities, Sri Lanka exhibited a clear mortality gradient across educational groups. Further, the high and rising relative inequalities in under 5 mortality in Sri Lanka showed that the achievement of low mortality might be at the expense of increasing relative mortality inequalities between socioeconomic groups. Increasing inequalities in malnutrition and undercoverage of health care, perhaps related to a strong gradient in female autonomy across educational groups, may have contributed to the rising relative under 5 mortality inequalities in this country.

Lower urinary tract symptoms; an undetected cause for morbidity in adults.

INTRODUCTION: Lower urinary tract symptoms (LUTS) have been recognised as a cause of distress among adult men and women. In view of the increasing elderly population in Sri Lanka, the number of individuals with LUTS is likely to increase. OBJECTIVES: To determine the prevalence and severity of LUTS in a community sample of older men and women residing in an urban area. DESIGN: Cross-sectional descriptive study. SETTING: Field practice area of National Institute of Health Sciences, Kalutara. METHODS: Households were selected using probability proportionate to the size (PPS) method. 985 adult men and women (above 40 years of age) were interviewed by Public Health Midwives regarding LUTS using an interviewer-administered questionnaire. The questions were adapted from ICSmale questionnaire and the Bristol Female Lower Urinary Tract questionnaire. RESULTS: 209 (47%) men and 338 (62.6%) women reported at least one urinary symptom. In men, urgency, nocturia, dysuria and frequency were the most prevalent symptoms with stress incontinence being the least prevalent. Majority of the symptoms showed an increase in prevalence with age. In women, dysuria, stress incontinence, urgency and frequency were the most prevalent symptoms. Hesitancy, intermittency, and stress and urge incontinence were the most bothersome symptoms among men, and urge and stress incontinence among women. CONCLUSIONS: Prevalence of LUTS was high in our study population.

Adenocarcinoma of the stomach in a tertiary care hospital in Sri Lanka.

OBJECTIVES: Adenocarcinoma of the stomach carries a dismal prognosis when it presents late. Our objective was to describe the location of the tumour, stage at presentation, resectability and survival in a cohort of patients with adenocarcinoma of the stomach, presenting to a tertiary referral centre. DESIGN AND SETTING: Data were collected retrospectively from all patients with gastric neoplasms who presented to the University Surgical Unit, Colombo South Teaching Hospital from May 2000 to October 2006. RESULTS: Ninety three patients presented with malignant gastric neoplasms during this period. Majority (86/93) were adenocarcinomas. Mean age at presentation was 58.3 (range 33-83) years. Male to female ratio was 3.15:1. 52.6% of tumours were in the proximal stomach involving the cardia. Thirty per cent involved the body, and 17.5% the distal stomach. 93% had Stage III or IV disease at presentation. Twenty seven patients (32.5%) had resectable tumours with a mean survival of 25.3 months. Five year survival was less than 5%, in patients who were not offered surgical resection. There were no patients in our series with early gastric cancer. CONCLUSION: All our patients presented with advanced gastric cancer and the majority had unresectable disease. The high proportion of patients having proximal gastric carcinoma is similar to the recent changes seen in the west.